Combined Bimolecular Fluorescence Complementation (BiFC) and Förster Resonance Energy Transfer (FRET) Reveals Ternary SNARE Complex Formation in Living Plant Cells

نویسندگان

  • Ralph Panstruga
  • Mark Kwaaitaal
  • Nana F. Keinath
  • Simone Pajonk
  • Christoph Biskup
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Breakthrough Technologies Combined Bimolecular Fluorescence Complementation and Förster Resonance Energy Transfer Reveals Ternary SNARE Complex Formation in Living Plant Cells

Various fluorophore-based microscopic methods, comprising Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation (BiFC), are suitable to study pairwise interactions of proteins in living cells. The analysis of interactions between more than two protein partners using these methods, however, remains difficult. In this study, we report the successful application of ...

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Combined bimolecular fluorescence complementation and Forster resonance energy transfer reveals ternary SNARE complex formation in living plant cells.

Various fluorophore-based microscopic methods, comprising Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation (BiFC), are suitable to study pairwise interactions of proteins in living cells. The analysis of interactions between more than two protein partners using these methods, however, remains difficult. In this study, we report the successful application of ...

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تاریخ انتشار 2010